Figure 5.

MiR-222-3p inhibitor relieves DSS-induced damage of colon. (A) qRT-PCR analysis of miR-222-3p in the IECs from DSS-induced mice. (B) DAI scores. (C) CMDIs. (D) Colon images. (E) Colon length. (F~I) morphological observation with hematoxylin and eosin (H&E)-stained colon sections as indicated, and arrows indicate ulcer healing sites. (J~M) Immunohistochemical analyses of BRG1 expression in colon tissues, and arrows indicate IECs locations. Data are presented as the median (P25, P75) (n = 8) in (C) and mean ± SD in (A, B, E) (n = 8). ^* P < 0.05, ^** P < 0.01, ^*** P < 0.001. Normal control: Normal control group; UC: UC group; AAV-NC: UC+ AAV-negative control group; AAV-222-3p inhibitor: UC+ miR-222-3p inhibitor group. N.S., no significance. Scale bar: 100 µm