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. 2023 Feb 9;14:726. doi: 10.1038/s41467-023-36236-2

Fig. 3. SET1 complexes support gene expression through an interaction with WDR82.

Fig. 3

a A schematic illustrating the SET1A domains that were tethered to the reporter gene. b A bar plot showing the mean fold induction of reporter gene expression following tethering of the GFP, full length SET1A (FL), NTD, RRM, Linker, and the NSET-SET (SET) domain fragments to the reporter gene. Error bars represent SEM from seven biological replicates. c A multiple sequence alignment of the N-terminal domains of SET1A (1A) or SET1B (1B) from the indicated species. The red box highlights the conserved and invariant DPR motif. d An immunoprecipitation (IP) of the NTD of WT SET1A or the DPR/AAA mutant followed by western blot for WDR82. HDAC1 functions as a loading control for the input samples and a negative control for interaction with SET1A. e A bar plot showing the mean fold induction of reporter gene expression following tethering of GFP, WT-NTD, or the DPR/AAA-NTD of SET1A. Error bars represent SEM from seven biological replicates.