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. 2023 Feb 9;13:2337. doi: 10.1038/s41598-023-29559-z

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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No detectable change in steady-state neprilysin protein levels or activity in Sst-deficient App^NL-F/NL-F mice. (A) Sst gene knockout had no effect on steady-state neprilysin protein levels in extracts from mid-sagittal cut half brains of App^NL-F/NL-F mice. The lower panel depicts a Coomassie stain of the SDS-PAGE-separated brain extract fractions used in these analyses whose total protein levels had been adjusted by bicinchoninic acid analysis. (B) Sst gene knockout had no significant effect on neprilysin activity present in brain extracts from mid-sagittal cut half brains of App^NL-F/NL-F mice toward an internally quenched 5-carboxyfluorescein (5-FAM) conjugated neprilysin substrate. Whereas the upper chart shows the neprilysin activities within Sst wild-type and deficient App^NL-F/NL-F brain extracts relative to each other (normalized to the mean neprilysin activity measured in one of the Sst-deficient App^NL-F/NL-F mice), the lower chart depicts their mean neprilysin activities relative to the activity of a known amount of recombinant human neprilysin. Original western blots and their Coomassie-stained images are presented in Supplementary Fig. 3.