Figure 6.

Ribo-seq data provides evidence that the transcripts coding for the SUMO alpha isoforms are translated. Five different Ribo-seq studies chosen at random from the NCBI databases were mined to search for the sequences corresponding to exon-exon junctions specific for the SUMO1V3, SUMO2V2, and SUMO3V2 transcripts shown under “Pattern searched.” For each sequence, the nucleotides presented with clear background correspond to the upstream exon, whereas the underlined nucleotides shown with a gray background correspond to the downstream exon. The Sequence IDs for the datasets containing hits, as well as the number of hits on each sequence dataset, are shown, as are the total number of hits found and the likely independent hits. Three out of the studies mined gave no hits and correspond to study accession IDs SRP122522, SRP362491, and SRP286677. Notice however that shortening the sequences used to mine the datasets by two additional nucleotides (one at the 5’ end and one at the 3’ end) resulted in an increase in the number of hits and all studies giving at least one hit, with the sole exception of study accession ID SRP362491, which still gave no hits.