Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Feb 9;14:731. doi: 10.1038/s41467-023-36409-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023, corrected publication 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 3 — A Representative laser Doppler images of the legs on day –1 (before surgery), 0 (immediately after surgery), 7, 14, 21, and 28. Right, quantification of blood flow recovery after hindlimb ischemia as determined by the ratio of foot perfusion of the ischemic (left) and non-ischemic (right) legs in CTR and dKO mice treated with or without ACEI. n = 6. B Assessment of capillary density by IHC analysis using CD31⁺ staining in the ischemic gastrocnemius muscle. Bottom, quantification of CD31⁺ vessels per mm² in CTR and dKO mice treated with or without ACEI. n = 6. Scale bar: 50 μm. C Images of the Matrigel plugs retrieved from CTR or dKO mice treated with or without ACEI and immunofluorescence staining of CD31⁺ vessels in the Matrigel plugs. Right, quantification of vessel density and mRNA expression levels of CD31 and VE-cadherin assessed using qPCR analysis. n = 6. Scale bars: 1 cm (row 1) and 20 μm (row 2). Two-way ANOVA followed by Bonferroni multiple comparison analysis was performed for A–C. Data are presented as mean ± SEM. Source data are provided as a Source Data file.