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. Author manuscript; available in PMC: 2023 Oct 1.
Published in final edited form as: Prog Neurobiol. 2022 Jul 14;217:102316. doi: 10.1016/j.pneurobio.2022.102316

Figure 6.

Figure 6.

RNAseq characterization of iPSC lines generated in this study.

(A) Purified RNA of cell lysates collected from iNs DIV21 were analyzed by RNAseq. Genes were selected that mark subsets of neuronal and glial cells and a heat map was created using the pheatmap package in R. HKG, housekeeping gene iPSC/NPC, induced pluripotent stem cell/neural progenitor cell. (B) Differentially expressed genes (DEGs) in fADcorr ELAVL4 KO iNs compared to fADcorr iNs (C) DEGs in fADcorr iNs in which ELAVL4 was overexpressed (combined analysis of ELAVL4 sv1 and sv2 OE) compared to fADcorr iNs. (D) DEGs from the rescue experiments: the DEGs in fADcorr ELAVL4 KO iNs with ELAVL4 sv1+sv2 rescue compared to fADcorr ELAVL4 KO iNs. DEGs with FDR corrected P-values < 0.05 and fold changes ≥ |1.20| are indicated in red. Volcano DEGs plots were generated using the EnhancedVolcano package in R.