| Reagent / resource | Reference / source | Identifier /catalog number |
|---|---|---|
| Experimental models | ||
| CD1 feeder cells | charles river | CD‐1® IGS Mouse |
| G4 ES cells | George et al (2007) | RRID:CVCL_E222 |
| C57BL/6N | Jackson Laboratories | |
| General Reagents | ||
| 4sU | Merck Chemicals GmbH | #T4509 |
| TRIzol | Life Technologies GmbH | #15596018 |
| IAA | Merck Chemicals GmbH | #11149 |
| Cell culture Reagents | ||
| DMEM | Gibco | #10829‐018 |
| FCS | Biochrom | #S0615 |
| Glutamine | Lonza | #BE17‐605E |
| Penicillin/streptomycin | Lonza | #DE17‐603 |
| Nonessential amino acids | Gibco | #11140‐35 |
| 10 mM β‐mercaptoethanol | Sigma | #M‐7522 |
| Nucleosides | Chemicon | #ES‐008D |
| Murine Leukemia Inhibitory Factor | Chemicon | #ESG1107 |
| PBS | Lonza | #BE17‐512F |
| Trypsin | Gibco | #25300‐054 |
| Zebrafish experiments – Reagents | ||
| Dextran fluorescein | Thermo Fisher Scientific | #7136 |
| HBSS | Life Technologies GmbH | #14175095 |
| TryplE | Life Technologies GmbH | #A1217701 |
| Native Bacillus licheniformis Protease | Creative Enzymes | #NATE‐0633 |
| EDTA | Molekula | #900493 |
| BSA | Sigma‐Aldrich | #A4503 |
| DPBS | Gibco | #14190144 |
| Adult mouse cardiomyocytes – Reagents | ||
| Stock perfusion buffer | Rudolph et al (2019) | |
| 2,3‐Butanedionemonoxime | Rudolph et al (2019) | |
| Glucose | Sigma‐Aldrich | #G7528 |
| Perfusion buffer pH 7.46 | Rudolph et al (2019) | |
| Liberase blendzyme 1 | Roche | #1988417 |
| Trypsin | Gibco/Invitrogen | #15090046 |
| Bovine calf serum | HyClone | #SH30073 |
| Embryonic mouse cardiomyocytes – Reagents | ||
| Trypsin‑EDTA | Thermo Fisher Scientific | #25200056 |
| Reconstituted fluorescent reactive dye | Thermo Fisher Scientific | #L34955 |
| Bovine calf serum | HyClone | #SH30073 |
| Alexa Fluor 647 Rat Anti‐Mouse VCAM‐1 | BD Biosciences | #561612 |
| Mouse hippocampus – Reagents | ||
| Adult Brain Dissociation Kit | Miltenyi Biotec | #130‐107‐677 |
| Narcorene | Merial GmbH | |
| Heparin‐Natrium‐250,000‐ratiopharm | Ratiopharm | |
| Calcein AM dye | BD Pharmingen | #564061 |
| SSC | Carl Roth GmbH | #1054.1 |
| BSA | Sigma‐Aldrich | #A4503 |
| SuperRNase IN | Life Technologies GmbH | #AM2694 |
| DTT | Carl Roth GmbH | #6908.1 |
| Bulk RNA extraction | ||
| GlycoBlue | Life Technologies GmbH | #AM9516 |
| Chloroform | Carl Roth GmbH | #3313.1 |
| Isopropanol | Carl Roth GmbH | #9781.1 |
| Ethanol | Carl Roth GmbH | #5054.1 |
| Bulk RNA IAA treatment | ||
| DMSO | Sigma‐Aldrich | #D8418 |
| Ethanol | Carl Roth GmbH | #5054.1 |
| Na2PO4 | Alfa Alsar | #J60825 |
| DTT | Carl Roth GmbH | #6908.1 |
| Bulk library preparation | ||
| PCR primers | Junker et al (2014) | |
| First Strand buffer | Life Technologies GmbH | #18064014 |
| DTT 100 mM | Life Technologies GmbH | #707265 |
| Nuclease free water | Sigma | #W3513 |
| RNaseOut | Life Technologies GmbH | #10777019 |
| Superscript II | Life Technologies GmbH | #18064014 |
| Second‐strand buffer | ThermoFisher | #10812014 |
| DNA ligase | New England Biolabs | #M0205L |
| DNA Pol I | New England Biolabs | #M0209L |
| RNase H | New England Biolabs | #M0297L |
| AMPpure XP beads | Beckman Coulter | #19245300 |
| HiScribe T7 kit | New England Biolabs | #E2040S |
| Exo I | New England Biolabs | #M0293L |
| rSAP | New England Biolabs | #M0371L |
| Tris‐Acetat pH8.1 | Carl Roth GmbH | #71251 |
| Potassium‐Acetat | VWR Chemicals | #43763N |
| Mg‐Acetat | Carl Roth GmbH | #HN11.1 |
| RNAclean XP beads | Beckman Coulter | #18816300 |
| NEBNext PCR mix 2× | New England Biolabs | #M0541L |
| Methanol fixation | ||
| Methanol | Th. Geyer | #1437.2511 |
| HBSS | Life Technologies GmbH | #14175095 |
| Single cell library preparation | ||
| Single Cell 3' v3.1 kit | 10× Genomics | #1000121 |
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