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. 2023 Jan 26;23(3):993–1002. doi: 10.1021/acs.nanolett.2c04479

Figure 2.

Figure 2

Helper lipid charge influences LNP in vivo tropism at the cellular level. (a) LNPs were formulated to carry a DNA barcode and Cre mRNA; 137 LNPs were pooled into their respective libraries and were then administered to Ai14 mice. After 4 days, tdTomato signal was quantified and tdTomato+ cells were isolated. Next-generation sequencing identified LNPs that functionally transfected cells in vivo. (b) Normalized delivery of all LNPs across all cell types in each library. The control, unencapsulated barcode, delivered less efficiently than barcodes delivered by LNPs. tdTomato+ signal in (c) lung, (d) liver, (e) spleen, (f) heart, and (g) kidney cells. These data suggest preferential delivery to lung cell types by the cationic LNPs; no delivery was observed by the neutral and anionic LNP pools to lung cell types. ****P < 0.0001, ***P < 0.0006, **P < 0.01, *P < 0.03, one-way ANOVA, average ± SEM. (h) Normalized delivery as a function of hydrodynamic diameter.