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Top LNP from cationic screen (Cat-LNP) potently delivers aVHH mRNA to lung ECs. (a) Unsupervised clustering partitioned the lung into clusters of endothelial cells, fibroblasts, epithelial cells, and immune cell subtypes. (b) Cat-LNP delivered aVHH mRNA to subtypes of lung ECs more than the control group (Neu-LNP, An-LNP, and PBS) at the 1 h time point. (c) The percentage of aVHH+ cells for each of the 27 clusters.
