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(A) Scheme of covalent HaloTag7 labeling with a fluorescent ligand. (B) Structure of silicon rhodamine (SiR) modified with chloroalkane (SiR-CA = SiR-Halo35). (C) Scheme of non-covalent HaloTag7 labeling with a fluorescent exchangeable HaloTag Ligand (xHTL). (D) Structure of SiR-xHTLs consisting of methylsulfonamide (S5) or trifluoromethylsulfonamide (T5) ligands attached to the rhodamine.
