Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Jan 30;145(5):3075–3083. doi: 10.1021/jacs.2c11969

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2023 The Authors. Published by American Chemical Society

Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

(A) Scheme of non-covalent dHaloTag7 labeling with a fluorescent xHTL. (B) Structure of SiR-xHTLs consisting of alkane-hydroxy (Hy4 and Hy5) ligands attached to rhodamines. (C) Structural analysis of the TMR-Hy5/dHaloTag7 complex (PDB-ID: 7ZIZ, 1.5 Å resolution). Magnification on the binding pocket (distances in Å). (D) Representative confocal images of live U2OS cells expressing H2B-HaloTag7 or H2B-dHaloTag7 labeled with annotated xHTLs. Scale bars: 10 μm. (E) Summarizing table of xHTL combinations for two-color live-cell fluorescence microscopy.