Table 1. Binding Properties of Rhodamine-xHTLs to HaloTag7.
| HaloTag7/ligand | KDa [nM] | konb [106M–1s–1] | koffc[s–1] | ||||
|---|---|---|---|---|---|---|---|
| TMR | S5 | 311 | (275–351) | 6.0 | (5.8–6.1) | 1.9 | (1.6–2.2) |
| CPY | S5 | 142 | (115–176) | 2.0 | (1.9–2.0) | 0.3 | (0.2–0.4) |
| SiR | S5 | 109 | (91–130) | 4.0 | (4.4–4.9) | 0.4 | (0.2–0.6) |
| TMR | T5 | 166 | (150–185) | 5.6 | (5.5–5.8) | 0.7 | (0.5–1.1) |
| CPY | T5 | 60 | (48–75) | 4.4 | (4.3–4.5) | 0.3 | (0.2–0.4) |
| SiR | T5 | 67 | (48–93) | 5.5 | (5.1–5.8) | 0.4 | (0.2–0.5) |
a
KD obtained from fluorescence polarization assay.
b
kon obtained from stopped-flow measurement.
c
koff was calculated from KD and kon. Errors provided as 95% confidence intervals.