Fig. 4.

Enhancer signatures in PFAs and H3K27M DMGs show high expression in astrocyte-like tumor cells. a Enriched gene ontology biological process (GO BP) gene sets (Y-axis Negative Log10 p value, X-axis Odds ratio) for genes associated with enhancers shared by PFAs and H3K27M DMGs. Shading of points is influenced by p-value (darker shade with lower p-values) and number of overlapping points. b Enriched gene ontology biological process (GO BP) gene sets (Y-axis Negative Log10 p value, X-axis Odds ratio) for genes associated with enhancers unique to H3K27M DMGs. c Enriched gene ontology biological process (GO BP) gene sets for genes (Y-axis Negative Log10 p value, X-axis Odds ratio) associated with enhancers unique to PFA ependymomas. d Comparison of expression of the shared enhancer signature by cell type in H3K27M DMGs [4] and PFAs [6]. Signature scores per cell were calculated by taking the mean of expression values for the genes in the signature. e Expression of the H3K27M DMG-specific enhancer signature by DMG cell type. f Expression of the PFA-specific enhancer signature by DMG cell type. Data in d–f analyzed by non-parametric Kruskal–Wallis test and 95% confidence intervals. AC-like, Astrocyte-like; OC-like, Oligodendrocyte-like; OPC-like, Oligodendrocyte precursor-like; CC- cell cycle; AE-like, Astroependymal-like; Epd-like, Ependymal-like; G2M, G to M cell cycle related; GP-like, Glialprecursor-like; ImmRe, Immune related; Metab, Metabolic; NPC-like, Neuronal precursor-like; NSC-like, Neuronal stem cell-like; S-phase, S-phase cell cycle