| Data collection |
| Magnification |
165000× |
| Voltage (V) |
300 |
| Electron exposure (e− Å−2) |
47 |
| Defocus range |
−3.9 to 1.0 |
| Pixel size (pre-refinement) (Å) |
0.253 |
| Pixel size (post-refinement) (Å) |
0.254 |
| Data processing |
| Motion correction |
RELION 3.1.1 |
| CTF estimation |
CTFFIND4.1 |
| Symmetry imposed |
I1 |
| Initial particle images |
85449 |
| Final particle images |
85411 |
| Map resolution (Å) |
2.21 |
| FSC threshold |
0.143 |
| Refinement |
| Protein atoms per asymmetric unit (mean B factor, Å2) |
4333 (21.9) |
| Solvation |
219 H2O/2 Mg2+
|
| R.m.s.d. from ideal, bond lengths (Å) |
0.11 |
| R.m.s.d. from ideal, bond angles (°) |
1.3 |
| Ramachandran outliers |
1 |
| Cross-correlation (model–map; grid points within 2.2 Å of atoms) |
0.871 |
| Envelope correction/equivalent overall B factor (for optimal fitting of model to sharpened map) (Å2) |
−4.3/−17.2 |
| Resolution from model–map refinement (RSRef
d
0.5) |
2.3 |
