Fig. 1.
The five diamonds on the flowchart contain the criteria used in this research for screening, allocation and inclusion/exclusion according to: (1) variant pathogenicity as per the list of diagnostic malignant hyperthermia (MH) mutations from the European Malignant Hyperthermia Group; (2) history (Hχ) of uneventful exposure to trigger anesthetics in relatives bearing a given mutation; (3) available data from at least two probands sharing a ryanodine receptor 1 (RYR1) mutation. VUSs, variants of unknown significance.