Figure 5.
Effects of POSTN on CRC cells. (a) Immunoblot analysis showing POSTN and ITGs in CRC and NIH/3T3 cells. (b) Results for FACS analyses showing ITGA6 and ITGB4 expression on CW-2 cells. (c–e) Results of migration assays showing that co-culture with NIH/3T3POSTN or addition of rPOSTN in the lower chamber accelerated the migration of CRC cells. Bar = 100 µm. Assays were performed in quadruplicate. Data are shown as mean ± S.D. *, p < 0.05, **, p < 0.01. (f) Addition of rPOSTN to the culture medium enhanced the proliferation of CRC cells. Assays were performed in triplicate. Data are shown as mean ± S.D. *, p < 0.05. (g) Immunoblot analysis showing upregulated P-FAK and P-AKT in rPOSTN-stimulated CRC cells. The uncropped bolts are shown in Supplementary Material File S1.