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. 2023 Feb 1;15(3):930. doi: 10.3390/cancers15030930

Figure 3.

Figure 3

USP45 promotes the proliferation, stemness and drug resistance of cervical cancer cells. (A–E) SiHa cells stably expressing plvx-puro vector (the negative control), USP45 or C199A were subjected to (A) Western Blot analyses, (B) tumorsphere formation assay, (C) FACS analyses for CD133-stained cells, (D) colony formation assay, (E) cells proliferation assay and (F,G) Hoechst 33,342 stain analysis. (H) The SiHa cells treated with increasing concentrations of doxorubicin (DOX) were subjected to cell viability assay. (I) The SiHa cells treated with 0.5 μM doxorubicin for 48 h were subjected to FACS analyses for Annexin V/PI-stained cells. Respective images and quantitation were shown. Data from independent experiments in duplicates were presented as means ± SD: p > 0.05 (no significance/ns), ** p < 0.01, *** p < 0.001, **** p < 0.0001. Scale bar is 100 μm in size. Experiments were conducted at least two times in duplicates.