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Localization of the satellites PGU-Sat-2 and PGU-Sat-3 in the chromosomes of P. guttatus. mi: the PGU-Sat-2 bearing microchromosome; ac: the acrocentric macrochromosome with both PGU-Sat-2 and PGU-Sat-3 signals; 2: chromosome 2; W: the W chromosome. (a,b) Merged images; (c,d) DAPI channel. Scale bar: 10 μm.
