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. 2023 Jan 31;15(3):893. doi: 10.3390/cancers15030893

Figure 2.

Figure 2

Stability of amino acid and metabolite levels in healthy donor blood stored at room temperature prior to plasma separation. Amino acids and metabolites related to metabolism of (A) Arg, (B) Gln, (C,D) Trp, (E) Phe and (F) Tyr. Amino acid and metabolite levels were determined by LC-MS/MS analysis and are expressed as mean ± SD of six individual donors. I3LA, I3AA, I3A and KynA are downstream metabolites of the unstable indole-3-pyruvic acid (I3P). One-way repeated measures ANOVA showed an overall effect of time on Arg, Orn, Glu, PP and 4HPP levels (all p < 0.001). Significant results of Dunnett’s multiple comparisons test with the first timepoint as reference group are indicated in the graphs. * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001. 4HPP: 4-hydroxyphenylpyruvic acid; Arg: l-arginine; Cit: l-citrulline; Gln: l-glutamine; Glu: l-glutamic acid; I3A: indole-3-aldehyde; I3AA: indole-3-acetic acid; I3LA: indole-3-lactic acid; Kyn: l-kynurenine; KynA: kynurenic acid; Orn: l-ornithine; Phe: phenylalanine; PP: phenylpyruvic acid; Trp: l-tryptophan; Tyr: l-tyrosine.