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. 2023 Jan 31;15(3):877. doi: 10.3390/cancers15030877

Figure 6.

Figure 6

Transient phosphorylation of mTOR/AKT and cJUN pathway with short-term anisomycin treatment and sustained phosphorylation of mTOR in long-term anisomycin-treated and p38–130429/160915 cells. (A): Protein expression of phospho-mTOR, AKT, p90, and cJUN with HSP90 as the loading control under untreated, DMSO, and 0.1 µM anisomycin treatment for 30 min and 24 h in cell line 130429. Densitometry plot represents the expression of proteins as the fold change in relation to HSP90 expression. (B): Protein expression of phospho-mTOR, AKT, p90, and cJUN with HSP90 as the loading control under untreated, DMSO, and 0.1 µM anisomycin treatment for 30 min and 24 h in cell line 160915. Densitometry plot represents the expression of proteins as the fold change in relation to HSP90 expression. (C): Protein expression of hosphor-mTOR, AKT, p90, and cJUN with HSP90 as the loading control under untreated, DMSO, and 0.1 µM anisomycin treatment for 30 min in EV control–130429 and p38–130429 cell lines. Densitometry plot represents the expression of proteins as the fold change in relation to HSP90 expression. (D): Protein expression of phospho-mTOR, AKT, p90, and cJUN with HSP90 as the loading control under untreated, DMSO, and 0.1 µM anisomycin treatment for 30 min in EV control–160915 and p38–160915 cell lines. Densitometry plot represents the expression of proteins as the fold change in relation to HSP90 expression.