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. 2023 Jan 31;15(3):877. doi: 10.3390/cancers15030877

Figure 7.

Figure 7

Transient suppression of autophagy by reduced conversion of LC3 with p38 activation. (A): Protein expression of LC3 with HSP90 as the loading control under untreated, DMSO, 0.1 µM anisomycin treatment for 30 min or 24 h, and 10 µM HCQ treatment for 24 h in cell line 130429. Densitometry plot represents the expression of proteins as the fold change in relation to HSP90 expression. (B): Protein expression of LC3 with HSP90 as the loading control under untreated, DMSO, 0.1 µM anisomycin treatment for 30 min or 24 h, and 10 µM HCQ treatment for 24 h in cell line 160915. Densitometry plot represents the expression of proteins as the fold change in relation to HSP90 expression. (C): Protein expression of p62 and LC3 with HSP90 as the loading control under untreated, DMSO, 0.1 µM anisomycin treatment or 24 h, and 10 µM HCQ treatment for 24 h in EV control–130429 and p38–130429 cell lines. Densitometry plot represents the expression of proteins as the fold change in relation to HSP90 expression. (D): Protein expression of LC3 with HSP90 as the loading control under untreated, DMSO, 0.1 µM anisomycin treatment for 30 min or 24 h, and 10 µM HCQ treatment for 24 h in EV control–160915 and p38–160915 cell lines. Densitometry plot represents the expression of proteins as the fold change in relation to HSP90 expression.