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. 2023 Feb 1;15(3):918. doi: 10.3390/cancers15030918

Figure 9.

Figure 9

Silencing of RIPK4 using siRNA has no impact on apoptosis or necroptosis in WM266.4 cells measured 48 h after transfection. (a) Transcript levels of BCL-2, BAD, MCL-1, RIPK1, RIPK3 48 h after transfection with RIPK4.si1 or neg.si normalized to GAPDH. ND—not detected. (b) Caspase activity 3/7. (c) Representative Annexin V-FITC/7AAD double staining and FACS analysis (left). Annexin V-FITC positive cells (sum of Ann+/7AAD- and Ann+/7AAD+; right). Each bar represents the mean ± SD of three biological replicates. All RIPK4.si transfected cells were compared with neg.si (scrambled control) samples. Statistical analysis was performed using the Student’s t-test. * p < 0.05, ** p < 0.01 was considered significant.