Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Jan 25;15(3):737. doi: 10.3390/cancers15030737

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Ripk4 tumor suppressor function is dependent on its kinase activity. (A) Representative image of tumors in Ripk4^fl/fl mice transduced with lentivirus (LV) carrying Cre recombinase and green fluorescent protein (GFP). Inlet picture shows the tumor with green fluorescence when imaged under a fluorescent microscope indicating the expression of GFP. (B) Representative mouse image of tumors in Pik3ca^H1047R;Ripk4^fl/fl mice transduced with lentivirus (LV) carrying Cre recombinase. (C) Tumor-free survival for LSL-Pik3ca^H1047R;Ripk4^+/+, LSL-Pik3ca^H1047R;Ripk4^fl/+, LSL-Pik3ca^H1047R;Ripk4^fl/fl mice transduced with LV-Cre (n ≥ 5 per group; p < 0.0001, log-rank test). (D) Western blot analysis of FLAG-tagged Ripk4 proteins (WT: wildtype or K51R: kinase-dead mutant) immuno-precipitated (IP) from primary mouse keratinocytes treated with phosphatase or left untreated. (E) Tumor-free survival for LSL-Pik3ca^H1047R;Ripk4^+/+ and LSL-Pik3ca^H1047R;Ripk4^fl/fl mice transduced either with LV-Cre-tdTomato or LV-Cre-WT Ripk4 or LV-Cre-Ripk4 kinase dead mutant (K51R) (n ≥ 5 per group; p < 0.0001, log-rank test).