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Detection of eryptosis by flow cytometry. Percentage of apoptotic RBCs positive to Annexin 5 and Trypan Blue detected in samples treated with 10 µM HgCl2 (4 h at 37 °C) with or without pre-treatment with 10 µM HT and/or HVA (1 h at 25 °C). ns, not statistically significant versus control; *** p < 0.001 versus left untreated (control); °°° p < 0.001 versus 10 µM HgCl2, one way ANOVA followed by Bonferroni’s post-hoc test (n = 11).
