Figure 2.

Ab-oligo amplification molar ratio titration. (A) MCF7-HER2 xenograft FFPE tissue sections were stained with either a HER2 primary antibody for indirect IF or a HER2 Ab-oligo conjugate (HER2 = yellow and DAPI = white), where a titration of molar excess of Amp IS to AmpS was performed from 1:1–160:1. (B) Negative controls for each sample were stained with only fluorophore-labeled secondary antibodies or the respective concentrations of AmpS and Amp IS without an Ab-oligo conjugate. (C) Image quantification was performed to calculate the relative signal-to-background ratio (SBR) for all staining conditions. Images shown are representative of multiple fields of view and quantification results are reported as a representative average. Scale bar = 50 μm.