Figure 5.

Optimized docking strand (DS) sequence design. (A) Three DS were evaluated for secondary structure formation and redesigned to reduce these potential structures. (B) Fixed cells were stained for HER2 using conventional indirect IF and HER2 Ab-oligo conjugates containing either the original or optimized KpnIB (green), SalIC (yellow), and PvuIIA (red) DS sequence. All images are set to auto-contrast settings for visualization of HER2-specific staining patterns. (C) Relative fluorescence intensity was quantified for each image for antibody staining comparison between indirect IF, the original DS sequence design, and the optimized DS sequence design. Images shown are representative of multiple fields of view and quantification results are reported as a representative average of all images. Scale bar = 50 μm.