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. 2023 Jan 24;15(3):705. doi: 10.3390/cancers15030705

Figure 2.

Figure 2

Deletion of GSK3B did not alter antitumor activity or maturation of FC-21 expanded NK cells. Both WT and GSK3B-KO NK cells were cocultured with Kasumi1 (AML tumor cell line) for 4 (A) or 220 h (B,C). “B” time course of long-term cell killing and “C” bar graph representative of indicated hours; (n = 3). (D–F) IL2, IFNγ and TNFα release was assessed after 4 h of WT and GSK3B-KO NK cells with PHA (n = 3). (G), CD57 expression was evaluated by flow cytometry on FC-21 expanded WT and GSK3B-KO NK cells (n = 6). (H), B3GAT1 expression in WT, FC-21 expanded WT, and FC-21 expanded GSK3B-KO NK cells as measured by RNA-seq. ns = non-significative, ** p ≤ 0.01, *** p ≤ 0.001. See also supplementary Figure S3 for cytotoxicity against other cancer cell lines, and supplementary Figure S4 for representative CD57 gating.