Figure 3.

GSK3B-KO in NK resulted in transcriptomic changes, RNA-seq analysis was performed on NK cells from FC-21 expanded WT and GSK3B-KO. (A) volcano plot representing DEG (WT/KO). (B) GO enrichment (WT). Heatmap (C) and RNA-seq boxplots (D) showing the top downregulated and top upregulated genes affected by GSK3B deletion. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001. For (C), rows are centered; unit variance scaling is applied to rows. Both rows and columns are clustered using correlation distance and average linkage.