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. 2023 Jan 29;15(3):819. doi: 10.3390/cancers15030819

Figure 5.

Figure 5

DHEA reduces the expression and secretion of the CCL5 in TNBC cells. (A) ELISA for C-C Motif Chemokine Ligand (CCL5) protein secretion in MDA-MB-231 and MDA-MB-436 breast cancer cells treated with vehicle (−) or DHEA 10 μM for 24 h. (B) Immunofluorescent staining of CCL5 protein expression in MDA-MB-231 and MDA-MB-436 treated as in (B) DAPI staining was used for nuclei detection (inset). (C) Boyden chamber transmigration assays in cells treated with vehicle (−), DHEA 10 µM with or without CCL5 recombinant protein (rh) 20 ng/mL for 24 h. (D) Trans-well recruitment of THP-1 cells in response to 1% charcoal-stripped media (−) and the conditioned medium (CM) derived from MDA-MB-436 breast cancer cells treated with vehicle (436-CM) or with DHEA 10 µM (DHEA-treated 436-CM) with or without CCL5 rh 20 ng/mL. * p < 0.05; ** p < 0.005; *** p < 0.0005.