Comparison between gene expression analysis of sex (male)-specific genes CYP2C11 (Panels (A,B), CYP3A18 (panels (C,D) and AR (Panels (E,F) by real-time PCR in male and female rat livers. Male rats, panels (A,C,E), subcutaneously treated with different doses of estradiol valerate plus cyproterone acetate five times a week, for 2 weeks: CM 0—sesame oil, D1M 0.09 + 0.33, D2M 0.09 + 0.93, and D3M 0.18 + 0.33 mg. Female rats, panels (B,D,F), subcutaneously treated with different doses of testosterone enanthate two times a week, for 2 weeks: CF 0—sesame oil, D1F 0.45, D2F 0.95, and D3F 2.05 mg. Data are presented as mean ∆∆Ct values ± standard deviation, with control samples as calibrators and GAPDH as the reference gene. Statistical significance: * p < 0.05 Mann–Whitney test.