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. 2023 Feb 2;13(3):559. doi: 10.3390/diagnostics13030559

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Amino acid substitutions within the Omicron variant lineage. Black color represents shared mutations, red Omicron BA.1, blue BA.2, orange BA.3, and green BA.4/BA.5. BA.4 and BA.5 share a similar spike profile as BA.2, except for additional mutations 69–70del, L452R, F486V (green) and reversions to wild type Q493 (Q493R in BA.1, BA.2, and BA.3). BA.4 and BA.5 differ from each other by three amino acid mutations outside Spike. BA.4 additional mutations: ORF7b:L11F, N:P151S. BA.5 additional mutations: M:D3N [35].