Table 1.

PLS-DA classification results for the four comparisons investigated: N° of variables and LVs included in each model, %Accuracy (%Acc) and NER%, both in fitting and cross-validation, for the models built on raw data or on first derivative data. Cross-validation was applied with 6 cancellation groups taking out at the same time all the replications of the samples analyzed the same day (1 control and 1 treated fish, 6 total measurements).

		Raw Data				First Derivative
		N° Variables	N° LV	%Acc	NER%	N° Variables	N° LV	%Acc	NER%
Muscle Short Term	Fitting	38	10	86.11	86.11	61	5	100	100
	Cross-validation			83.33	83.33			100	100
Muscle Long Term	Fitting	558	6	100	100	314	1	100	100
	Cross-validation			100	100			100	100
Skin Short Term	Fitting	470	7	100	100	273	2	100	100
	Cross-validation			100	100			100	100
Skin Long Term	Fitting	294	7	100	100	108	3	100	100
	Cross-validation			100	100			100	100