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. 2023 Jan 25;12(3):410. doi: 10.3390/cells12030410

Figure 1.

Figure 1

HK expression remained relatively stable in ApoE2- and ApoE3-expressing cells; in contrast, a gradual decrease with increasing passages occurred in ApoE4-expressing cells. (a) ApoE protein expression levels in human ApoE2-, ApoE3-, or ApoE4-expressing stable cell lines at P1. Results were normalized to the ApoE3 no. 16 line. n = 3 repeats/line. Three lines that showed similar ApoE levels (ApoE2 no. 6, ApoE3 no. 16, ApoE4 no. 13; indicated by arrows) were chosen and used in the following studies. (b–d) ApoE, HK1, and HK2 exhibited similar levels in the three chosen cell lines at P5. Results were normalized to the ApoE3 group. n = 4–7 repeats/line. (e–h) HK1 and HK2 protein levels progressively declined in ApoE4-expressing cells; however, ApoE and GLUT4 expression levels were unchanged in all three ApoE cells lines, from P7 to P15. Results were normalized to the P7 group. n = 2–6/group. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 vs. P15 group, # p < 0.05, ## p < 0.01, #### p < 0.0001 vs. P13 group, and & p < 0.05 vs. P7 group. N.S.: not significant.