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. 2023 Jan 25;12(3):410. doi: 10.3390/cells12030410

Figure 2.

Figure 2

HK was upregulated in ApoE2-expressing cells and downregulated in ApoE4-expressing cells, which occurred concurrently with downregulation of PFKP and PKM1 by ApoE2 and upregulation by ApoE4. (a–c) Protein levels of both isoforms and activity of HK were upregulated in ApoE2-expressing cells and downregulated in ApoE4-expressing cells at all three passages, P9, P11, and P13. n = 5–9/group. (d,e) PFKP and PKM1 protein levels were significantly downregulated in ApoE4-expressing cells compared to ApoE2- and ApoE3-expressing cells. n = 4–7/group. (f) GLUT1, GLUT3, and GLUT4 protein levels showed no significant differences among the three ApoE cell lines at P11. n = 5–8/group. Results were normalized to the ApoE3 group in each passage. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. N.S.: not significant.