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. 2023 Jan 25;12(3):410. doi: 10.3390/cells12030410

Figure 5.

Figure 5

Expression of ApoE2 ameliorated HK deficits and improved glycolytic metabolism in ApoE4-expressing cells. (a) N2a-hApoE4 stable cells were transfected with human ApoE2 plasmids and maintained for 48–72 h. Cell lysates were harvested and analyzed for ApoE2 protein expression to confirm the transfection efficiency. n = 5/group. (b–d) Protein levels of both HK1 and HK2, and HK activity, were significantly higher in ApoE4 cells transfected with ApoE2 (ApoE4 + ApoE2) compared to ApoE4 cells transfected with empty vector (ApoE4 + V). n = 2–5/group. (e) Glycolytic readouts from a Seahorse stress test were markedly increased in ApoE4 + ApoE2 cells compared to ApoE4 + V cells. n = 8/group. (f–i) N2a-hApoE4 stable cells were transfected with human ApoE2 or ApoE3 plasmids and maintained for 48–72 h. Expression of ApoE2 but not ApoE3 increased HK expression in ApoE4-expressing cells. n = 3–7/group. Results were normalized to the ApoE4 + V group. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.