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. 2023 Feb 2;12(3):486. doi: 10.3390/cells12030486

Figure 5.

Figure 5

D-BHB promotes an increase in lysosomal proteins and lysosomal number. (A) Representative Western blot and quantification of LAMP2/β-actin and (B) CTSB/pro-CTSB. (C) Representative micrographs of NADH autofluorescence and lysotracker in neurons treated and non-treated with 5 mM D-BHB for 24 h. Graph shows quantification of lysosome particles in neurons treated or non-treated with D-BHB. Data are normalized with respect to control. Data are expressed as Mean ± SEM from 5 (A) or 4 (B,C) independent experiments. Data in (A,B) and were analyzed by one-way ANOVA followed by Fisher’s post-hoc test. * p < 0.05 vs. control. Data in C were analyzed by Student’s t-test. * p < 0.05 vs. control.