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. 2023 Feb 2;12(3):486. doi: 10.3390/cells12030486

Figure 7.

Figure 7

Nuclear localization of FOXOs and TFEB transcription factors in cortical neurons treated with D-BHB. Analysis of nuclear or cytoplasmic localization by subcellular fractionation of FOXO3a (A), FOXO1 (B) and TFEB (C). Representative immunoblot (left) and quantification (right) are shown. Histone H1.0 was used as a nuclear loading control, and GAPDH was used as a cytoplasmic loading control. (D) Expression of autophagy and lysosomal genes detected by qRT-PCR in cortical neurons exposed to 5 mM of D-BHB for 24 or 48 h. Data are expressed as Mean ± SEM of 4 (A–C) or 3 (D) independent experiments. Data were analyzed by one-way ANOVA followed by Fisher’s post-hoc test. * p < 0.05 vs. control.