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. 2023 Feb 2;12(3):486. doi: 10.3390/cells12030486

Figure 9.

Figure 9

SIRT2 mediates the neuroprotective effect of D-BHB against energy stress. Diagram of the experimental design to test the neuroprotective effect of D-BHB pre-incubation using the mitochondrial uncoupler CCCP (A) or GD/GR in cortical neurons (D). MTT reduction (B,E) and LDH activity in culture medium (C,F) of cortical neurons pre-incubated 24 or 48 h with 5 mM D-BHB followed by 24-h exposure to 3 µM CCCP (B,C), or 2 h GD followed by 24 h GR (E,F). (G) Diagram of the experimental design to test the effect of SIRT2 inhibition by AGK2 on neuroprotection of D-BHB against energy stress induced by GD/GR in cortical neurons. (H) Reduction of MTT and (I) LDH activity of cortical neurons pre-incubated 24 h with 5 mM D-BHB with or without AGK2 (5 μM) followed by 2 h GD and 24 h GR. (J) Diagram of the experimental design to test the neuroprotective effect of NAD pre-incubation against GD/GR. (K) Reduction of MTT and (L) LDH activity. NAD (5 mM) was pre-incubated for 24 h followed by 2 h GD and 24 h GR. Data are expressed as Mean ± SEM of 3 (B,C), 6 (E,F,H,I) or 4 (K,L) independent experiments. Data were analyzed by one-way ANOVA followed by Fisher’s post-hoc test. * p < 0.05 vs. control, & p < 0.05 vs. GD/GR, # p < 0.05 vs. D-BHB.