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List of reagents added to imaging chamber, with indication of wash (−) or incubation time.
| Step | Reagent | Volume (μL) | Incubation time (minutes) |
|---|---|---|---|
| 1 | Neutravidin | 7.5 | 5 |
| 2 | MB-cold | 10 | - |
| 3 | κ-casein | 7.5 | 2 |
| 4 | MB-warm | 10 | - |
| 5 | Biotinylated microtubule (diluted in MB-warm) | 10 | 10 |
| 6 | MB-warm | 10 | - |
| 7 | Warm κ-casein | 7.5 | 2 |
| 8 | 2 nM PRC1 diluted in κ-casein | 10 | 5 |
| 9 | Non-Biotinylated Microtubule | 10 | 10 |
| 10 | MB-warm x 2 | 10 | - |
| 11 | Assay mix | 10 | - |
| Attached seeds are stable for around 20 minutes at this point | |||
