. Author manuscript; available in PMC: 2023 Feb 18.

Published in final edited form as: J Vis Exp. 2022 Feb 18;(180):10.3791/63377. doi: 10.3791/63377

Table 5: Soluble Tubulin Mix Components.

Mix at start of experiment and keep on ice.

Reagent	Volume (μL)
Recycled tubulin, 10 mg/mL	10
MB-Cold	10.3
MBMC	13.7
BRB80-DTT	3.4
GTP, 10 mM	6.7
ATP, 10 mM (If using kinesins)	6.7
Fluorescently labeled tubulin, 10 mg/mL	1 (Resuspend lyophilized labeled tubulin in cold BRB80-DTT)