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. 2023 Jan 19;11(3):310. doi: 10.3390/healthcare11030310

Figure 5.

Figure 5

Targets involved into regulation of the cell cycle, down-regulated in HAECs after 24 h treatment with elution fraction. (a) Biological process categories overrepresented in 119 down-regulated genes in HAECs after 24 h treatment with eluted proteins (array data, n = 2). Significance generated with ConsensusPathDB-human (release 34, [26]) is presented on the x-axis as negative logarithmized p-value. (b) Validation of down-regulated protein targets with immunoblotting (n = 3). Proteins of interest were detected in cell lysates obtained from untreated HAECs (Ctrl), HAECs treated with 0.125 µg/mL of elution (E), 0.125 µg/mL of serum (S). Samples from three independent experiments are marked as Exp 1, Exp 2, Exp 3. KIF20A band is shown with the arrow. (c) Signal intensities from protein targets were normalized to signal intensity from β-actin, signals from treated cells are expressed as percent from untreated control. Data are presented as mean ± SEM. (* p ≤ 0.05; ** p ≤ 0.001).