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. 2023 Feb 3;12(3):660. doi: 10.3390/foods12030660

Figure 4.

Figure 4

Construction and validation of Area silencing vector. (a) Construction strategy of Area silencing vector; (b) PCR validation of pSt-Area-R vector, ck is water, pst is empty vector, 1 and 2 are pSt-Area-R; (c) validation of double digestion (StuⅠ and XhoI) on pSt-Area-R vector, pst is pSilent1 vector, 1 and 2 are pSt-Area-R; (d) PCR validation of pSt-Area-L vector (primer PSR/PSF), ck is water, pst is pSilent1 vector, 1 is pSt-Area-L; (e) validation of double digestion (HindⅢ and XhoⅠ) on pSt-Area-L vector, pst is empty vector, 1 is pSt-Area-L.