Table 1.

Immunohistochemistry-staining protocol.

Primary Antibody	Primary Antibody Dilution	HIER Method	Antigen Retrieval Time	Antigen Retrieval Temp.	Peroxidase Blocking	Primary Antibody Incubation	HRP Incubation
Rabbit monoclonal HIF-1α antibody, clone EP1215Y, Abcam	1:500	Hot air oven	30 min	100 °C Tris EDTA pH = 9.0	15 min	40 min	40 min