Skip to main content
. Author manuscript; available in PMC: 2024 Feb 28.
Published in final edited form as: J Mol Biol. 2023 Jan 6;435(4):167946. doi: 10.1016/j.jmb.2023.167946

Figure 2: Rad5-catalyzed fork reversal with different fork DNA substrates.

Figure 2:

A, Plot showing the increase of FRET as a function of time on a fork DNA substrate containing no gap. B, Plot showing the increase of FRET as a function of time on a fork DNA substrate containing a 10-nucleotide gap on the leading arm. C, Plot showing the increase of FRET as a function of time on a fork DNA substrate containing a 10-nucleotide gap on the lagging arm. D, Close up of the initial linear region of panel A. The solid line represents the best fit of the data with an initial rate of 0.13 nM/min. E, Close up of the initial linear region of panel B. The solid line represents the best fit of the data with an initial rate of 4.6 nM/min. F, Close up of the initial linear region of panel C. The solid line represents the best fit of the data with an initial rate of 1.9 nM/min. Mean and standard deviations of the initial rates are given in the text.