Figure 1.

Halothane causes AiN in mitochondrial mutants. Flies of three genotypes at 11 to 13 days of age were exposed to two hours of 1.5% HAL administered by a carrier gas containing between zero and 75% O₂ and corresponding concentrations of N₂. The number of dead flies was counted 24 h after exposure. Panel (A) shows the percent mortality under the various conditions while panel (A’) shows the difference in percent mortality between flies exposed to halothane and those exposed to O₂ alone. Data shown as mean ±SEM, n indicates number of biological replicates of 25–30 mixed-sex flies contained in a single conical 50 mL tube, each shown as dots. ns: not significant, *: p < 0.05, ***: p < 0.001, ****: p < 0.0001. Note: high rates of natural attrition in mutants.