Figure 2.

AiN in ND-23⁶⁰¹¹⁴ flies is age-dependent. Groups of flies were exposed for two hours to 2% ISO in 75% O₂ at weekly intervals. Mortality was determined 24 h after exposure. No mortality attributable to anesthetic exposure was observed in Canton S flies while ND-23⁶⁰¹¹⁴ experienced an abrupt increase in mortality at two weeks of age (A). (A’): same data as A with control mortality subtracted (Δ Mortality). Each dot represents a biological replicate of 15–35 mixed-sex flies. Results are presented as mean ± SEM. Dashes indicate that the experiment was not performed because there were fewer than three vials of 15 flies.