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Hyperoxic ISO increases 24 h mortality in ND-SGDHG6447 mutants. (A). Flies were exposed to 2% ISO in 75% O2 for two hours at 15–20 days old, returned to culture vials and scored 24 h after exposure. Mortality increased in mutants but not in wild-type flies (p = 0.0254, unpaired t-test). (A’). Data as in A, control mortality subtracted (Δ Mortality). Each dot represents a biological replicate of 15–35 mixed-sex flies. Results presented as mean ± SEM. * p < 0.05.
