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. 2023 Jan 27;9:972433. doi: 10.3389/fvets.2022.972433

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Copyright © 2023 Zhang, Zhang, Zhang, Liu, Wang, Wang, Zou, Hu, Lian, Lu and Bai.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Establishment of IFN-ε SYBR fluorescence quantitative polymerase chain reaction. (A,B) Standard curve based on different dilution gradients of plasmids. (C) Standard curve for the correlation between copy number and cycle threshold value.