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. 2023 Jan 27;9:972433. doi: 10.3389/fvets.2022.972433

Figure 5.

Figure 5

Co-expression of pET28-small ubiquitin-like modifier (SUMO)-IFN-ε and Tf16 chaperone plasmid, protein purification and Western blotting. (A) Expression of (SUMO)-IFN-ε and Tf16 chaperone plasmid and protein purification. Lanes: 1, Recombinant strain containing pET28a-SUMO-MiIFN-ε and Tf16 chaperone protein plasmid cultured; 2, cultured and induced for 48 h using L-arabinose; 3, Induced with isopropylβ-d-thiogalactoside for 48 h at 16 °C; 4, cell lysate supernatant; 5, centrifugal precipitation after cell lysis; 6, Elution buffer; 7, the purified SUMO-IFN-ε was digested using a SUMO protease to remove the SUMO label; M, protein molecular weight standard. (B) Western blot analysis of His-tag recombinant SUMO-MiIFN-ε by 6-poly histidine monoclonal antibodies. 1 and 2. SUMO-MiIFN-ε;M, protein molecular weight standard.