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Histology and immunostaining of the TES produced with (A–F) Surge SFM or with (A’–F’) ckDME-Ham. Epidermal layers are identified (sb: stratum basale, ss: stratum spinosum, sg: stratum granulosum, sc: stratum corneum). (A,A’) Masson’s trichrome staining. Immunofluorescence staining against (B,B’) filaggrin, (C,C’) transglutaminase 1 (TG1), (D,D’) keratin 14 (K14), (E,E’) Ki-67, and (F,F’) type IV collagen (Col IV). Cell nuclei were stained with Hoechst reagent. Scale bars: 50 µm.
